Combined Anti-influenza Virus Effect of Synthetic and Natural Viral Inhibitors
نویسندگان
چکیده
The combined virus-inhibitory effects of a proteinaceous protease inhibitor, produced by Streptomyces sp. 225b (BF), rimantadine hydrochloride (Rim), ε-aminocaproic acid (ACA) and a polyphenol extract from Geranium sanguineum L. (PC) have been investigated on the reproduction of influenza virus A/Germany/34, strain Rostock (H7N1) in MDCK cell cultures. The simultaneous application of BF and PC, a plant preparation with established anti-influenza-virus activity, led to antagonistic virus-inhibitory effect. The combined use of BF and Rim, a selective anti-influenza drug, in doses, which by themselves do not suppress significantly viral replication, resulted in an additive increase of inhibition. The combinations BF+АСА, both of the preparations being proteolytic inhibitors, limited viral replication in a synergistic fashion. The results support the efficacy of combined chemotherapy; however to achieve a synergistic enhancement of the virusinhibitory effect it is essential to precisely select the doses of the individual components in the combinations. Introduction The∗ combined use of antiviral agents helps the enhancement of viral inhibition, the reduction of toxicity and the prevention of antiviral resistance. The application of natural and synthetic viral inhibitors in appropriate combinations also offers possibilities in this direction. This paper presents the results from the investigation of the combined virus-inhibitory effects of two synthetic inhibitors (rimantadine hydrochloride and ε-aminocaproic acid) and two inhibitors of natural ∗Abbreviation: BF protease inhibitor, produced by Streptomyces sp. 225b; CPE cytopathogenic effect; ACA ε-aminocaproic acid; IVY Infectious virus yield reduction assay; KIU Kunitz inhibitory units; MDCKMadin-Darby canine kidney cells; PC polyphenol extract from Geranium sanguineum L.; Rim rimantadine hydrochloride; TCID50 50% tissue culture infectious doses. origin (a protease inhibitor, produced by Streptomyces sp. 225b and a plant polyphenol extract). Materials and Methods Substances: Fermentation product from Streptomyces sp. 225b (BF): the strain is from the collection of the Department of Microbiology, Sofia University; the cultivation conditions and the partial purification were as in (1). Polyphenol extract from Geranium sanguineum L. (PC): the plant material and extraction were as in (7). Rimantadine hydrochloride (Rim) was purchased from Hoffman La Roche Inc., Nutley, NJ, USA. ε-aminocaproic acid (ACA) was a gift from Prof. V.P. Lozitsky, Odessa, Anti-plague Institute, Ukraine. Cells and Virus: MDCK cells, media for cultivation and influenza virus A/chicken/Germany/34, strain Rostock (H7N1) (A/Rostock) were as described in (1). Infectious virus yield (IVY) reduction
منابع مشابه
New Anti-Influenza Agents: Targeting the Virus Entry and Genome Transcription
Introduction: The emergence and spread of the pandemic H1N1 influenza virus in 2009 indicates a limitation in the strategy to control the infection, despite a long-established vaccination programme and approved antivirals. Production the proper vaccine against influenza is difficult due to the genetic recombination of virus in the event of pandemic and co-circulation of drug-resistance variants...
متن کاملAnti-influenza Activity of a Novel Polyoxometalate Derivative (POM-4960)
There are many effective chemothereutic agents used in influenza disease which some of them inhibit virus replication by interfering with FluV (influenza virus) viral binding or its penetration into cell membrane. A series of polyoxometalates compounds such as POM-523 and PM-504 have been synthesized and have showed inhibitory effects on viruses. In this study we examined anti influenza activit...
متن کاملThe Potential Effect of Glycyrrhiza Glabra on Early Step of Influenza Virus Replication
Background and Aims: The emergence of drug-resistant influenza viruses has become a serious threat for human and animal populations. Glycyrrhiza glabra (Gg) is a traditional medicine clinically used for the treatment of viral respiratory infection symptoms in most countries. We evaluated the effects of the herb on influenza virus replication in human lung cultured cells (A549) following the det...
متن کاملPomegranate peel extract inhibits internalization and replication of the influenza virus: An in vitro study
Objective: Influenza virus, which is associated with high level of morbidity and mortality, has been recently considered a public health concern; however, the methods of choice to control and treat it are limited. Our previous study showed anti-influenza virus activity of pomegranate peel extract (PPE). In this study, the mechanism through which PPE acts against influenza virus...
متن کاملThe Combined Use of rHVT-H5 and rHVT-F Vector Vaccines in the Hatchery Enhances Immunity against Highly Pathogenic Avian Influenza H5N1 and Velogenic Newcastle Disease Viral Infections in Commercial Chickens
Highly pathogenic avian influenza H5N1 and Newcastle disease viral infections cause severe illness in chickens and vaccination is a strategic tool of controlling these diseases. Hence, this study was conducted to evaluate the efficacy of using both recombinant herpesvirus of turkey (rHVT-H5 and rHVT-F) vector vaccines at day-old, in the hatchery, under field conditions. Vaccinated chickens were...
متن کامل